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Restriction enzymes

Restriction enzymes cut DNA at a specific base sequence or region of a DNA strand. They are ideal for genetic experiments where we want to isolate genes for expression to work out what they do for the cell.

Restriction endonucleases are enzymes that cleave DNA at specific palindromic sequences approximately six nucleotides long. They cleave the DNA unevenly leaving ‘sticky ends’ that can be used to ligate pieces of DNA together.